Part:BBa_K1911002:Design

ClpP from E. coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The ClpP sequence which was pulled from the E. coli genome contained illegal restriction enzyme sites. In order to remove these illegal sites, wobbles were introduced into the ClpP sequence so that the gene is not cut anywhere and is allowed to function properly.

Source

ClpP is a naturally present gene in the E. coli genome. The ClpP sequence was pulled from the E. coli genome.